Re: GFP-actin mouse - where can I get it?
Posted by
Michael Schell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-actin-mouse-where-can-I-get-it-tp3463212p3463549.html
In model species such as yeast, GFP-actin does not rescue an actin knockout. In a number of mammalian systems, GFP-actin does not behave identically to actin (although some monomer is incorporated into filaments). Thus, the GFP-actin mouse may have had the GFP-actin driven by a promoter weak enough to not swamp out endogenous actin. I wonder if they had trouble maintaining a mouse line with "just right" amounts of GFP-actin expression. This is only a guess. Wasn't a line of GFP-actin mice generated in Switzerland?
Michael
Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD 20814-3220
tel: (301) 295-3249
[hidden email]
>>> Waldo Schmidt <
[hidden email]> 08/17/09 8:58 PM >>>
Hello all,
For a while I have been contemplating using a GFP-actin mouse as I knew it
existed.
However, as I draw to finding this mouse, I do not succeed.
I have tried to communicate with the researchers who developed the HUGE
GFP actin mouse, but have no response as yet.
Are there any other sources of this mouse?
Kind regards