FISH measurement

Posted by Boswell, Carl A - (cboswell) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FISH-measurement-tp3480943.html

Hi all,
I have a user who wants to measure the volume, not intensity, of the
FISH-labeled region in a z-series.

Any issues with the following proposal, besides using a histogram to get
pixel numbers?

1. Threshold the FISH signals for control and experimental Z-Series
images with identical conditions.
2. Create a histogram for each thresholded image and use total pixel number
as
the FISH signal volume.

Thanks,
C

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]