Re: FISH measurement
Posted by
JOEL B. SHEFFIELD on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FISH-measurement-tp3480943p3481098.html
It seems to me that you first have to be sure that each z-slice is independent --that you are not detecting fluorescence from one slice in another.--
Joel
On Thu, Aug 20, 2009 at 2:31 PM, Carl Boswell
<[hidden email]> wrote:
Hi all,
I have a user who wants to measure the volume, not intensity, of the FISH-labeled region in a z-series.
Any issues with the following proposal, besides using a histogram to get pixel numbers?
1. Threshold the FISH signals for control and experimental Z-Series
images with identical conditions.
2. Create a histogram for each thresholded image and use total pixel number as
the FISH signal volume.
Thanks,
C
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
--
Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail:
[hidden email]
URL:
http://astro.temple.edu/~jbs