Re: FISH measurement

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FISH-measurement-tp3480943p3481179.html

Sounds reasonable; in effect you are counting how many voxels are
above a particular threshold.  Once you have the number of voxels, if
you need an actual measurement just multiply by the real-world volume
of one voxel.

Craig


On Thu, Aug 20, 2009 at 12:31 PM, Carl
Boswell<[hidden email]> wrote:

> Hi all,
> I have a user who wants to measure the volume, not intensity, of the
> FISH-labeled region in a z-series.
>
> Any issues with the following proposal, besides using a histogram to get
> pixel numbers?
>
> 1. Threshold the FISH signals for control and experimental Z-Series
> images with identical conditions.
> 2. Create a histogram for each thresholded image and use total pixel number
> as
> the FISH signal volume.
>
> Thanks,
> C
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]
>