Re: FISH measurement

Posted by McDonald, David L on Aug 20, 2009; 7:40pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/FISH-measurement-tp3480943p3481251.html

Carl,

I believe the ImageJ 3D Objects Counter plugin will give the volume
of each object after thresholding the image.

Dave

Dave McDonald
Scientific Imaging Lab
Fred Hutchinson Cancer Research Center
1100 Fairview Avenue North, DE-512
Seattle, WA 98109
206-667-4205
http://www.fhcrc.org


At 11:31 AM 8/20/2009, you wrote:

>Hi all,
>I have a user who wants to measure the volume, not intensity, of the
>FISH-labeled region in a z-series.
>
>Any issues with the following proposal, besides using a histogram to
>get pixel numbers?
>
>1. Threshold the FISH signals for control and experimental Z-Series
>images with identical conditions.
>2. Create a histogram for each thresholded image and use total pixel number as
>the FISH signal volume.
>
>Thanks,
>C
>
>Carl A. Boswell, Ph.D.
>Molecular and Cellular Biology
>University of Arizona
>520-954-7053
>FAX [phone]