Posted by
Cameron, Lisa on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Recommendations-for-commercial-multi-photon-system-purchase-tp3499069p3499627.html
Bogdan,
Are you considering pre-churp on the IR laser?
Did you actually compare the Olympus 25x objective and the Leica 20x?
I have not compared them myself, but have heard raves about the Olympus 25x.
How many NDD channels will you need? the difference in 2 NDDs vs. 4 NDDs should
be significant in price, so for the 2 systems you mentioned, I would say the
Olympus is a better price. When you are comparing pricing, you need to compare
equivalent features/parts. Also if you are leaning at all toward the Leica, I
would seriously consider an AOBS - this is the technology that separates them
from the others.
I do not have personal experience with in vivo rodent brain imaging, but it is
my understanding that an upright microscope would be the recommended
configuration. But, if you need to do confocal imaging on live cells, I guess an
inverted would be the best.
I recently went through a similar process of evaluation of systems and would be
happy to compare notes offline or over the phone.
- Lisa
[hidden email]
-----Original Message-----
From: Confocal Microscopy List on behalf of Bogdan Stoica
Sent: Sun 8/23/2009 12:11 PM
To:
[hidden email]
Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial multi-photon
system purchase
Hi fellows,
I am also in the last stages of choosing a confocal/multiphoton system for
our laboratory. After seeing multiple demos i am thorn between an Olympus
FV1000MPE system and an Leica SP5 II. Our needs are to be able to do both
confocal images on fixed and live in vitro cells and live in vivo rodent
brain imaging. Both systems come with a UV, visible Argon four lines (488
etc), laser diode 560 and 633 and IR laser (Chameleon for Leica and Maitai
for Olympus), a complete set of Apo objectives as well as the famous 25x NA
1.05 from Olympus and the "equivalent" 20x from Leica. The microscopes are
inverted and the system also includes 2 NDD for Leica and 4 NDD in the case
for Olympus.
Fro what I could see the Leica software appeared more user friendly and I
would give their system a better feel for confocal imaging and manufacturers
potential upgrades- the system I am pricing does not include the AOBS
feature, "white laser" or resonant scanner but those could be added later.
On the other hand the Olympus seems a better choice for multiphoton imaging
and an easier system to tinker with. The tech support and managers for both
companies seemed outstanding to me here in the Washington DC-Baltimore area.
I found interesting that I was not able to find any info on "how much" such
a system should cost and after intense "negotiations" the quites range on my
case around the ~700k mark with Olympus a little bellow and Leica a little
above.
I was wondering if people who recently had to make a similar decision can
offer me some guidance both on the merits of their systems once they were
installed as well as on the price, support etc.
Thank you very much,
Bogdan
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