Re: Recommendations for commercial multi-photon system purchase

Posted by Bogdan Stoica on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Recommendations-for-commercial-multi-photon-system-purchase-tp3499069p3499902.html

Dear Lisa,

Thank you for your kind response.
 Let me adress the issue you raised in turn:
-I did not get the chance to compare side by side the Olympus 25x vs. Leica
20x. I could only met with people that had rave reviews about the 25x so I
would tend to five Olympus a slight advantage in this regard.
-initially both systems had 4 NDDs but Leica had to leave only 2 to meet our
budget. I saw people that having 4 NDDs coupled to 2 IR lasers and I was
told me that even with one laser as in our case more NDDs could be
beneficial for recording other channels such as intrinsic fluorescence etc.
Again an extra point for Olympus.
-You are right to point that for Leica features such as the AOBS as well as
white laser should be unique advantages. The problem is that they would put
the system outside our immediate budget although the fact that these
upgrades are available in the future including as on-side upgrades for the
AOBS justify an extra advantage for Leica.
-Indeed, the need to do live cell imaging is directing us toward an inverted
system.
-the IR laser are the Maitai deepsee for olympus and the Chameleon Ultra II
for the Leica.

Bogdan