http://confocal-microscopy-list.275.s1.nabble.com/Re-Recommendations-for-commercial-multi-photon-system-purchase-tp3499069p3500025.html
with you directly. Please let me know if there is a good time to call
> Bogdan,
>
> Are you considering pre-churp on the IR laser?
>
> Did you actually compare the Olympus 25x objective and the Leica 20x?
> I have not compared them myself, but have heard raves about the Olympus 25x.
>
> How many NDD channels will you need? the difference in 2 NDDs vs. 4 NDDs should
> be significant in price, so for the 2 systems you mentioned, I would say the
> Olympus is a better price. When you are comparing pricing, you need to compare
> equivalent features/parts. Also if you are leaning at all toward the Leica, I
> would seriously consider an AOBS - this is the technology that separates them
> from the others.
>
> I do not have personal experience with in vivo rodent brain imaging, but it is
> my understanding that an upright microscope would be the recommended
> configuration. But, if you need to do confocal imaging on live cells, I guess an
> inverted would be the best.
>
> I recently went through a similar process of evaluation of systems and would be
> happy to compare notes offline or over the phone.
>
> - Lisa
>
>
[hidden email]
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List on behalf of Bogdan Stoica
> Sent: Sun 8/23/2009 12:11 PM
> To:
[hidden email]
> Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial multi-photon
> system purchase
>
> Hi fellows,
>
> I am also in the last stages of choosing a confocal/multiphoton system for
> our laboratory. After seeing multiple demos i am thorn between an Olympus
> FV1000MPE system and an Leica SP5 II. Our needs are to be able to do both
> confocal images on fixed and live in vitro cells and live in vivo rodent
> brain imaging. Both systems come with a UV, visible Argon four lines (488
> etc), laser diode 560 and 633 and IR laser (Chameleon for Leica and Maitai
> for Olympus), a complete set of Apo objectives as well as the famous 25x NA
> 1.05 from Olympus and the "equivalent" 20x from Leica. The microscopes are
> inverted and the system also includes 2 NDD for Leica and 4 NDD in the case
> for Olympus.
> Fro what I could see the Leica software appeared more user friendly and I
> would give their system a better feel for confocal imaging and manufacturers
> potential upgrades- the system I am pricing does not include the AOBS
> feature, "white laser" or resonant scanner but those could be added later.
> On the other hand the Olympus seems a better choice for multiphoton imaging
> and an easier system to tinker with. The tech support and managers for both
> companies seemed outstanding to me here in the Washington DC-Baltimore area.
> I found interesting that I was not able to find any info on "how much" such
> a system should cost and after intense "negotiations" the quites range on my
> case around the ~700k mark with Olympus a little bellow and Leica a little
> above.
> I was wondering if people who recently had to make a similar decision can
> offer me some guidance both on the merits of their systems once they were
> installed as well as on the price, support etc.
> Thank you very much,
>
> Bogdan
>
>
>
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