Posted by
Roman Veith on
Aug 26, 2009; 2:04pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslip-on-coverslip-can-cells-inbetween-be-imaged-tp3517025p3517126.html
Hi Waldo,
we use custom made coverslip holder built in our toolshop. This works
very well for us. Cells grow on coverslips and whenever needed single
coverslips with cells are collected from the cell culture. The coverslip
holder is simple a metal device with an adapter for coverslips in the
middle. Imagine the metal device as a metal object slide with a hole in
the middle, designed in a way, that the coverslip exactly fits into it,
without passing through. E.g. for a 18x18 coverslip the hole has
dimensions of ~16x16. Another piece of metal with a ~16 circular whole
in it and a rubber seal is placed on top. Immidiately after transfer of
the coverslips new buffer is added. The rubber seal of the upper device
assures that everything is leak-proof. Maybe after this procedure some
recovery time for the cells is appropriate.
Cells can then be imaged even with an oil immersion objective - if
refractive index mismatch is no problem...
Regards,
Roman
Waldo Schmidt schrieb:
> We have the problem of our cells differentiating well on specific coverslips,
> and not those of mattek dishes. In the meantime of trying to identify other
> dishes, we continue to use coverslips.
>
> We image just with a dipping lens, but would like some more resolution.
>
> Is it possible to mount the "coverslip with cells" upsidedown onto a new
> mattek dish and then image it using an inverted microscope with a water
> immersion lens?
>
> Is there another way around this?
>
> Regards
>
--
--
Roman Veith
Dipl.-Biol.
Institut für physikalische und
theoretische Chemie
AG Biophysikalische Chemie
Universität Bonn
Wegelerstraße 12
53115 Bonn
Tel-Nr.: 0228-733089
Fax-Nr.: 0228-739424
http://www.thch.uni-bonn.de/pc/kubitscheck/