Re: Recommendations for commercial multi-photon system purchase

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Recommendations-for-commercial-multi-photon-system-purchase-tp3499069p3517343.html

Thanks for the clarification!  It's good to know you have the
provision for a direct laser port.

Craig


On Wed, Aug 26, 2009 at 1:52 AM, <[hidden email]> wrote:

>
> COMMERCIAL VENDOR RESPONSE
>
> Craig,
>
> just to clarify: in the Leica TCS SP5, the Multiphoton laser does not pass
> the AOBS or AOTF. There is a separate dedicated IR beam splitter port. So
> there are no dispersion issues due to the pulsed IR beam passing through the
> acousto-optical tuneable elements.
>
> Best regards
> Martin
>
> --------------------------------------------------------
> Martin Hoppe, Ph.D.
> Head of Market Management &
> Confocal Application Management
> Life Science Division
>
> Leica Microsystems CMS GmbH
> Am Friedensplatz 3 | 68165 Mannheim (Germany)
> Phone : [phone] | Fax : [phone]
> Cell: [phone]
>
>
>
>
>
> I've used the Leica white laser system during a demo.  It's nice, but
>
> its main purpose is for core facilities where a user could walk in
>
> with any arbitrary dye combination.  If you know what dyes you will be
>
> using on a regular basis, just pick fixed laser lines to address them.
>
> Regarding the multiphoton, if you are using a system that has an AOBS
>
> or the like in it, you could have dispersion issues with the pulsed
>
> laser.  I'd recommend getting one of the ones like the DeepSee with
>
> built-in dispersion control in that case.
>
> As for the objective lenses, apparently the 25x was designed
>
> specifically for laser scanning applications, but I'd want to see a
>
> side-by-side comparison with the 20x to be sure.
>
>
>
> Craig
>
>
> -----Original Message-----
> From: Craig Brideau <[hidden email]>
> To: [hidden email]
> Sent: Mon, Aug 24, 2009 5:02 pm
> Subject: Re: Recommendations for commercial multi-photon system purchase
>
> I've used the Leica white laser system during a demo.  It's nice, but
>
> its main purpose is for core facilities where a user could walk in
>
> with any arbitrary dye combination.  If you know what dyes you will be
>
> using on a regular basis, just pick fixed laser lines to address them.
>
> Regarding the multiphoton, if you are using a system that has an AOBS
>
> or the like in it, you could have dispersion issues with the pulsed
>
> laser.  I'd recommend getting one of the ones like the DeepSee with
>
> built-in dispersion control in that case.
>
> As for the objective lenses, apparently the 25x was designed
>
> specifically for laser scanning applications, but I'd want to see a
>
> side-by-side comparison with the 20x to be sure.
>
>
>
> Craig
>
>
>
> On Sun, Aug 23, 2009 at 2:03 PM, Bogdan Stoica<[hidden email]>
> wrote:
>
>> Dear Lisa,
>
>>
>
>> Thank you for your kind response.
>
>>  Let me adress the issue you raised in turn:
>
>> -I did not get the chance to compare side by side the Olympus 25x vs.
>> Leica
>
>> 20x. I could only met with people that had rave reviews about the 25x so I
>
>> would tend to five Olympus a slight advantage in this regard.
>
>> -initially both systems had 4 NDDs but Leica had to leave only 2 to meet
>> our
>
>> budget. I saw people that having 4 NDDs coupled to 2 IR lasers and I was
>
>> told me that even with one laser as in our case more NDDs could be
>
>> beneficial for recording other channels such as intrinsic fluorescence
>> etc.
>
>> Again an extra point for Olympus.
>
>> -You are right to point that for Leica features such as the AOBS as well
>> as
>
>> white laser should be unique advantages. The problem is that they would
>> put
>
>> the system outside our immediate budget although the fact that these
>
>> upgrades are available in the future including as on-side upgrades for the
>
>> AOBS justify an extra advantage for Leica.
>
>> -Indeed, the need to do live cell imaging is directing us toward an
>> inverted
>
>> system.
>
>> -the IR laser are the Maitai deepsee for olympus and the Chameleon Ultra
>> II
>
>> for the Leica.
>
>>
>
>> Bogdan
>
>>
>
>