Hi Craig,
We are thinking of building a semi-home built 2-photon
confocal system ourselves. Could you please tell me a little more about your
system.
Thanks,
Neeraj.
Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Craig Brideau
Sent: Wednesday, August 26, 2009 10:58 AM
To: [hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase
We have a semi-home-built combination 2-photon/confocal
system which
saw a lot of use. We bought a dedicated confocal (Nikon
C1Si) to
offload the confocal users from the 2-photon system.
There was so
much demand for both modes that there were many scheduling
conflicts.
It's much better to have dedicated systems if at all
possible.
Craig
On Wed, Aug 26, 2009 at 6:27 AM, Guy
Cox<[hidden email]> wrote:
> It is entirely possible to avoid a coverslip on an
inverted microscope - we just build a dam of dental moulding silicone around a
dipping lens and fill it with water.
>
> We bought a Leica femtosecond 2-photon system back
in 2000 - but the patent never applied to Australia. (That did mean it had to
be put together here not in Germany). My understanding is that Leica have
now taken out a licence for what little time remains on the Cornell patent so
that you can now buy a femtosecond system anywhere. Actually you always
could, you just had to do the integration yourself, since it was always the deal
that no action would be taken against labs building a home-brew system.
But building a home-brew system isn't for everyone - and now there's no
need for that.
>
> I agree 100% that it is crazy to put every function
under the sun on one scope - that's a recipe for huge conflicts of interest.
>
>
Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor &
Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351
7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Sylvie Le Guyader
> Sent: Wednesday, 26 August 2009 7:10 PM
> To: [hidden email]
> Subject: Re: Recommendations for commercial
multi-photon system purchase
>
> Hi Bogdan
>
> We have recently gone through the purchase of a two
photon/confocal microscope. We opted for the Zeiss710 upright with a spectral
detector and for buying an extra confocal inverted for our cell work. When
using an inverted microscope, you offset the first plane you can image by the
thickness of the coverslip (160um) which is a real pity.
>
> You must also consider that many lenses that are
dedicated for 2 photon imaging do not correct chromatic aberration in the
visible range which means that you will need another objective for confocal
imaging with more than 1 fluorophore.
>
> Does anyone know if Leica now supports femtosec
pulsed lasers? They have so far bypassed the Zeiss/Biorad patent by slowing
down the laser to picosec but I think the patent recent stopped. A psec laser
would mean less penetration which could be a problem for you on your inverted
microscope, especially if you want to image highly scattering samples.
>
> Our experience (we have paid dearly before learning
that) is that it is much better to wait a bit longer for more money to come in
and get a second dedicated system. Piling up functions on one system often ends
up compromising all of them. Several companies (Zeiss, La Vision) offer
dedicated two photon systems that are a lot cheaper than two photon/confocal
combined and that are excellent for two photon imaging. If the system you are
looking at has both single and two photon lasers and if you also need two sets
of objectives, you are you are paying for all the expensive parts twice anyway.
>
> Good luck!
>
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader
> Dept of Biosciences and Nutrition
> Karolinska Institutet
> Novum
> 14157 Huddinge
> Sweden
> +46 (0)8 608 9240
>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List on behalf of
Bogdan Stoica
>> Sent: Sun 8/23/2009 12:11 PM
>> To: [hidden email]
>> Subject: Re: [CONFOCALMICROSCOPY]
Recommendations for commercial
>> multi- photon system purchase
>>
>> Hi fellows,
>>
>> I am also in the last stages of choosing a
confocal/multiphoton system
>> for our laboratory. After seeing multiple demos
i am thorn between an
>> Olympus FV1000MPE system and an Leica SP5 II.
Our needs are to be able
>> to do both confocal images on fixed and live in
vitro cells and live
>> in vivo rodent brain imaging. Both systems come
with a UV, visible
>> Argon four lines (488 etc), laser diode 560 and
633 and IR laser
>> (Chameleon for Leica and Maitai for Olympus), a
complete set of Apo
>> objectives as well as the famous 25x
> NA
>> 1.05 from Olympus and the "equivalent"
20x from Leica. The microscopes
>> are inverted and the system also includes 2 NDD
for Leica and 4 NDD in
>> the
> case
>> for Olympus.
>> Fro what I could see the Leica software appeared
more user friendly
>> and I would give their system a better feel for
confocal imaging and
> manufacturers
>> potential upgrades- the system I am pricing does
not include the AOBS
>> feature, "white laser" or resonant
scanner but those could be added later.
>> On the other hand the Olympus seems a better
choice for multiphoton
> imaging
>> and an easier system to tinker with. The tech
support and managers for
> both
>> companies seemed outstanding to me here in the
Washington DC-Baltimore
> area.
>> I found interesting that I was not able to find
any info on "how much"
> such
>> a system should cost and after intense
"negotiations" the quites range
>> on
> my
>> case around the ~700k mark with Olympus a little
bellow and Leica a
>> little above.
>> I was wondering if people who recently had to
make a similar decision
>> can offer me some guidance both on the merits of
their systems once
>> they were installed as well as on the price,
support etc.
>> Thank you very much,
>>
>> Bogdan
>>
>>
>>
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