Re: Coverslip on coverslip - can cells inbetween be imaged?

Posted by Pascal Lorentz-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslip-on-coverslip-can-cells-inbetween-be-imaged-tp3517025p3517465.html

Hi Waldo,

I think what Roman describes is also sold by a company in Switzerland.
They are called Life Imaging Services:
http://www.lis.ch/

No commercial interest, just a satisfied customer.

Best regards

Pascal


Roman Veith schrieb:

> Hi Waldo,
>
> we use custom made coverslip holder built in our toolshop. This works
> very well for us. Cells grow on coverslips and whenever needed single
> coverslips with cells are collected from the cell culture. The
> coverslip holder is simple a metal device with an adapter for
> coverslips in the middle. Imagine the metal device as a metal object
> slide with a hole in the middle, designed in a way, that the coverslip
> exactly fits into it, without passing through. E.g. for a 18x18
> coverslip the hole has dimensions of ~16x16. Another piece of metal
> with a ~16 circular whole in it and a rubber seal is placed on top.
> Immidiately after transfer of the coverslips new buffer is added. The
> rubber seal of the upper device assures that everything is leak-proof.
> Maybe after this procedure some recovery time for the cells is
> appropriate.
> Cells can then be imaged even with an oil immersion objective - if
> refractive index mismatch is no problem...
>
> Regards,
> Roman
>
>
> Waldo Schmidt schrieb:
>> We have the problem of our cells differentiating well on specific
>> coverslips, and not those of mattek dishes. In the meantime of trying
>> to identify other dishes, we continue to use coverslips.
>>
>> We image just with a dipping lens, but would like some more resolution.
>>
>> Is it possible to mount the "coverslip with cells" upsidedown onto a
>> new mattek dish and then image it using an inverted microscope with a
>> water immersion lens?
>> Is there another way around this?
>> Regards
>>  
>
>

Pascal Lorentz
Department of Biomedicine
University of Basel
Mattenstrasse 28
4058 Basel
Switzerland