http://confocal-microscopy-list.275.s1.nabble.com/Re-Recommendations-for-commercial-multi-photon-system-purchase-tp3499069p3518250.html
We use a Spectra Physics Tsunami. It's all manual so it can be tricky
seems to have given it a long lifespan. The lab bought it in ~2001,
the lab a few years ago. The laser is seven or eight years old now
and still quite happily chugging along. I've even managed to teach
the biology people how to tune it themselves @;-). The scan head had
them, but since then it's still a regular performer for our lab. You
Andrew Ridsdale, Ileana Micu, and Peter K. Stys
Applied Optics, Vol. 43, Issue 8, pp. 1669-1675 doi:10.1364/AO.43.001669
> Hi Craig,
>
> so what femtosecond laser you use in your semi-home-built system?
>
> here is one home-brew multiphoton system built at UCLA based on our Trestles
> lasers
http://www.dmphotonics.com/trestles%20brochure%20web.pdf>
>
http://www.youtube.com/watch?v=b0jmCqoHqkc&feature=channel_page>
> Best regards,
> Sergey Egorov
> Del Mar Photonics
> 4119 Twilight Ridge
> San Diego, CA 92130
> tel (858) 876-3133
> fax (858) 630-2376
> www.dmphotonics.com
>
[hidden email]
>
>> We have a semi-home-built combination 2-photon/confocal system which
>> saw a lot of use. We bought a dedicated confocal (Nikon C1Si) to
>> offload the confocal users from the 2-photon system. There was so
>> much demand for both modes that there were many scheduling conflicts.
>> It's much better to have dedicated systems if at all possible.
>>
>> Craig
>>
>> On Wed, Aug 26, 2009 at 6:27 AM, Guy Cox<
[hidden email]> wrote:
>>
>>>
>>> It is entirely possible to avoid a coverslip on an inverted microscope -
>>> we just build a dam of dental moulding silicone around a dipping lens and
>>> fill it with water.
>>>
>>> We bought a Leica femtosecond 2-photon system back in 2000 - but the
>>> patent never applied to Australia. (That did mean it had to be put together
>>> here not in Germany). My understanding is that Leica have now taken out a
>>> licence for what little time remains on the Cornell patent so that you can
>>> now buy a femtosecond system anywhere. Actually you always could, you just
>>> had to do the integration yourself, since it was always the deal that no
>>> action would be taken against labs building a home-brew system. But
>>> building a home-brew system isn't for everyone - and now there's no need for
>>> that.
>>>
>>> I agree 100% that it is crazy to put every function under the sun on one
>>> scope - that's a recipe for huge conflicts of interest.
>>>
>>> Guy
>>>
>>>
>>>
>>> Optical Imaging Techniques in Cell Biology
>>> by Guy Cox CRC Press / Taylor & Francis
>>>
http://www.guycox.com/optical.htm>>> ______________________________________________
>>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>> Electron Microscope Unit, Madsen Building F09,
>>> University of Sydney, NSW 2006
>>> ______________________________________________
>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>>> Mobile 0413 281 861
>>> ______________________________________________
>>>
http://www.guycox.net>>> -----Original Message-----
>>> From: Confocal Microscopy List [mailto:
[hidden email]]
>>> On Behalf Of Sylvie Le Guyader
>>> Sent: Wednesday, 26 August 2009 7:10 PM
>>> To:
[hidden email]
>>> Subject: Re: Recommendations for commercial multi-photon system purchase
>>>
>>> Hi Bogdan
>>>
>>> We have recently gone through the purchase of a two photon/confocal
>>> microscope. We opted for the Zeiss710 upright with a spectral detector and
>>> for buying an extra confocal inverted for our cell work. When using an
>>> inverted microscope, you offset the first plane you can image by the
>>> thickness of the coverslip (160um) which is a real pity.
>>>
>>> You must also consider that many lenses that are dedicated for 2 photon
>>> imaging do not correct chromatic aberration in the visible range which means
>>> that you will need another objective for confocal imaging with more than 1
>>> fluorophore.
>>>
>>> Does anyone know if Leica now supports femtosec pulsed lasers? They have
>>> so far bypassed the Zeiss/Biorad patent by slowing down the laser to picosec
>>> but I think the patent recent stopped. A psec laser would mean less
>>> penetration which could be a problem for you on your inverted microscope,
>>> especially if you want to image highly scattering samples.
>>>
>>> Our experience (we have paid dearly before learning that) is that it is
>>> much better to wait a bit longer for more money to come in and get a second
>>> dedicated system. Piling up functions on one system often ends up
>>> compromising all of them. Several companies (Zeiss, La Vision) offer
>>> dedicated two photon systems that are a lot cheaper than two photon/confocal
>>> combined and that are excellent for two photon imaging. If the system you
>>> are looking at has both single and two photon lasers and if you also need
>>> two sets of objectives, you are you are paying for all the expensive parts
>>> twice anyway.
>>>
>>> Good luck!
>>>
>>>
>>> Med vänlig hälsning / Best regards
>>>
>>> Sylvie
>>>
>>> @@@@@@@@@@@@@@@@@@@@@@@@
>>> Sylvie Le Guyader
>>> Dept of Biosciences and Nutrition
>>> Karolinska Institutet
>>> Novum
>>> 14157 Huddinge
>>> Sweden
>>> +46 (0)8 608 9240
>>>
>>>
>>>>
>>>> -----Original Message-----
>>>> From: Confocal Microscopy List on behalf of Bogdan Stoica
>>>> Sent: Sun 8/23/2009 12:11 PM
>>>> To:
[hidden email]
>>>> Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial
>>>> multi- photon system purchase
>>>>
>>>> Hi fellows,
>>>>
>>>> I am also in the last stages of choosing a confocal/multiphoton system
>>>> for our laboratory. After seeing multiple demos i am thorn between an
>>>> Olympus FV1000MPE system and an Leica SP5 II. Our needs are to be able
>>>> to do both confocal images on fixed and live in vitro cells and live
>>>> in vivo rodent brain imaging. Both systems come with a UV, visible
>>>> Argon four lines (488 etc), laser diode 560 and 633 and IR laser
>>>> (Chameleon for Leica and Maitai for Olympus), a complete set of Apo
>>>> objectives as well as the famous 25x
>>>>
>>>
>>> NA
>>>
>>>>
>>>> 1.05 from Olympus and the "equivalent" 20x from Leica. The microscopes
>>>> are inverted and the system also includes 2 NDD for Leica and 4 NDD in
>>>> the
>>>>
>>>
>>> case
>>>
>>>>
>>>> for Olympus.
>>>> Fro what I could see the Leica software appeared more user friendly
>>>> and I would give their system a better feel for confocal imaging and
>>>>
>>>
>>> manufacturers
>>>
>>>>
>>>> potential upgrades- the system I am pricing does not include the AOBS
>>>> feature, "white laser" or resonant scanner but those could be added
>>>> later.
>>>> On the other hand the Olympus seems a better choice for multiphoton
>>>>
>>>
>>> imaging
>>>
>>>>
>>>> and an easier system to tinker with. The tech support and managers for
>>>>
>>>
>>> both
>>>
>>>>
>>>> companies seemed outstanding to me here in the Washington DC-Baltimore
>>>>
>>>
>>> area.
>>>
>>>>
>>>> I found interesting that I was not able to find any info on "how much"
>>>>
>>>
>>> such
>>>
>>>>
>>>> a system should cost and after intense "negotiations" the quites range
>>>> on
>>>>
>>>
>>> my
>>>
>>>>
>>>> case around the ~700k mark with Olympus a little bellow and Leica a
>>>> little above.
>>>> I was wondering if people who recently had to make a similar decision
>>>> can offer me some guidance both on the merits of their systems once
>>>> they were installed as well as on the price, support etc.
>>>> Thank you very much,
>>>>
>>>> Bogdan
>>>>
>>>>
>>>>
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>>
>>
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