Re: Coverslip on coverslip - can cells inbetween be imaged?

Posted by Jacqueline Ross on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslip-on-coverslip-can-cells-inbetween-be-imaged-tp3517025p3519749.html

Hi Waldo,

I know others have already replied with some suggestions but I have one
more for you to consider. I recently bought two chambers from Quorum
Technologies, called Chamlide chambers. They are designed to take
various sizes of coverslip ( I bought the 25mm one) and are magnetic. I
have only just bought them having seen them at the Vancouver LiveCell
course and haven't yet tried them but they seem like they will work
really well.

No commercial interest!

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Waldo Schmidt
Sent: Thursday, 27 August 2009 1:31 a.m.
To: [hidden email]
Subject: Coverslip on coverslip - can cells inbetween be imaged?

We have the problem of our cells differentiating well on specific
coverslips,
and not those of mattek dishes. In the meantime of trying to identify
other
dishes, we continue to use coverslips.

We image just with a dipping lens, but would like some more resolution.

Is it possible to mount the "coverslip with cells" upsidedown onto a new

mattek dish and then image it using an inverted microscope with a water
immersion lens?

Is there another way around this?

Regards