Posted by
Alberto Diaspro on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslip-on-coverslip-can-cells-inbetween-be-imaged-tp3517025p3520052.html
Dear friends,
in my department at the Italian Institute of Technology there are 20
PhD positions open. A specific one is reported here.
The complete call can be found at www.iit.it.
All the best
Alby
----- Diaspro Lab call ----
Theme 3.1: Optical Nanoscopy and advanced high-resolution microscopy.
Tutor: Alberto Diaspro
A recognized advantage of optical microscopy lies in the fact that
allows non-invasive three-dimensional (3D)
imaging of live cells at the submicron scale with high specificity.
The advent of the visible fluorescent proteins
and of a myriad of fluorescent tags pushed fluorescence microscopy to
become the most popular imaging
tool in cell biology. The confocal and multiphoton versions of
fluorescence microscopy reinforce this
condition. However, like any other standard imaging system relying on
focused light, all these microscopes
are limited in spatial resolution because the smallest possible spot
size is imposed by diffraction. Several
approaches aimed at overcoming the diffraction limit. Stimulated
Emission Depletion (STED) microscopy is
one of the most promising Optical Nanoscopy approaches allowing an
ultimate resolution of 7.6 nm in the
optical regime. In STED microscopy, fluorescence emanating from the
periphery of the focused excitation
beam is suppressed by a second properly shaped beam that depletes the
excited state population through
stimulated emission. This effectively narrows fluorescent molecule
signature, the point spread function (PSF)
of the microscope, to permit superresolved images to be acquired. The
Optical Nanoscopy theme is related
to the development of an original STED architecture based in white
light laser illumination and to its
characterization for key applications to tracking of molecular events
in living cells towards the study of
degenerative processes like neuro-diseases, tumor progression and
nanoparticle toxic effects on biological
systems. Variations on the theme will include Fluorescence Photo-
Activation Light Microscopy (FPALM) and
Single Plane Illumination Microscopy (SPIM) approaches.
For further details concerning the research project, please contact:
[hidden email]
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Alberto Diaspro
Head, Nanophysics Unit
Senior Scientist
The Italian Institute of Technology -IIT
Via Morego, 30
16163 - Genova (Italy)
phone: +39 010 71781503
mobile: +393666719968
fax: +39 010 720321
http://www.iit.it[hidden email]
Professor of Applied Physics
Department of Physics
University of Genova
Via Dodecaneso, 33
16146 Genova - Italy
tel. +39 010 353 6426
fax. +39 010 314218
http://www.lambs.it[hidden email]
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