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Re: Coverslip on coverslip - can cells inbetween be imaged?

Posted by Seb Stephens on Aug 27, 2009; 1:54am
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslip-on-coverslip-can-cells-inbetween-be-imaged-tp3517025p3520752.html

Hi Jacqui,
 
waldo = me!!!!!!!!
 
I have just ordered these chamlide chambers too. You know theyre on special now? Like 50% off....
 
Speak soon


 
 
Sébastien Stephens
Harvard University School of Dental Medicine
188 Longwood Ave REB 313
Boston, MA 02115
Ph: 617-4327328
Fax: 617-4321897
[hidden email]



 

> Date: Thu, 27 Aug 2009 09:49:57 +1200
> From: [hidden email]
> Subject: Re: Coverslip on coverslip - can cells inbetween be imaged?
> To: [hidden email]
>
> Hi Waldo,
>
> I know others have already replied with some suggestions but I have one
> more for you to consider. I recently bought two chambers from Quorum
> Technologies, called Chamlide chambers. They are designed to take
> various sizes of coverslip ( I bought the 25mm one) and are magnetic. I
> have only just bought them having seen them at the Vancouver LiveCell
> course and haven't yet tried them but they seem like they will work
> really well.
>
> No commercial interest!
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
>
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland, NEW ZEALAND
>
> Tel: 64 9 373 7599 Ext 87438
> Fax: 64 9 373 7484
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Waldo Schmidt
> Sent: Thursday, 27 August 2009 1:31 a.m.
> To: [hidden email]
> Subject: Coverslip on coverslip - can cells inbetween be imaged?
>
> We have the problem of our cells differentiating well on specific
> coverslips,
> and not those of mattek dishes. In the meantime of trying to identify
> other
> dishes, we continue to use coverslips.
>
> We image just with a dipping lens, but would like some more resolution.
>
> Is it possible to mount the "coverslip with cells" upsidedown onto a new
>
> mattek dish and then image it using an inverted microscope with a water
> immersion lens?
>
> Is there another way around this?
>
> Regards


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