I've used the Leica white laser system during a demo. It's nice, but
its main purpose is for core facilities where a user could walk in
with any arbitrary dye combination. If you know what dyes you will be
using on a regular basis, just pick fixed laser lines to address them.
Regarding the multiphoton, if you are using a system that has an AOBS
or the like in it, you could have dispersion issues with the pulsed
laser. I'd recommend getting one of the ones like the DeepSee with
built-in dispersion control in that case.
As for the objective lenses, apparently the 25x was designed
specifically for laser scanning applications, but I'd want to see a
side-by-side comparison with the 20x to be sure.
Craig
I've used the Leica white laser system during a demo. It's nice, but
its main purpose is for core facilities where a user could walk in
with any arbitrary dye combination. If you know what dyes you will be
using on a regular basis, just pick fixed laser lines to address them.
Regarding the multiphoton, if you are using a system that has an AOBS
or the like in it, you could have dispersion issues with the pulsed
laser. I'd recommend getting one of the ones like the DeepSee with
built-in dispersion control in that case.
As for the objective lenses, apparently the 25x was designed
specifically for laser scanning applications, but I'd want to see a
side-by-side comparison with the 20x to be sure.
Craig
On Sun, Aug 23, 2009 at 2:03 PM, Bogdan Stoica<[hidden email]> wrote:
> Dear Lisa,
>
> Thank you for your kind response.
> Let me adress the issue you raised in turn:
> -I did not get the chance to compare side by side the Olympus 25x vs. Leica
> 20x. I could only met with people that had rave reviews about the 25x so I
> would tend to five Olympus a slight advantage in this regard.
> -initially both systems had 4 NDDs but Leica had to leave only 2 to meet our
> budget. I saw people that having 4 NDDs coupled to 2 IR lasers and I was
> told me that even with one laser as in our case more NDDs could be
> beneficial for recording other channels such as intrinsic fluorescence etc.
> Again an extra point for Olympus.
> -You are right to point that for Leica features such as the AOBS as well as
> white laser should be unique advantages. The problem is that they would put
> the system outside our immediate budget although the fact that these
> upgrades are available in the future including as on-side upgrades for the
> AOBS justify an extra advantage for Leica.
> -Indeed, the need to do live cell imaging is directing us toward an inverted
> system.
> -the IR laser are the Maitai deepsee for olympus and the Chameleon Ultra II
> for the Leica.
>
> Bogdan
>