Re: 3D volume rendering and measurement

Posted by Tim O'Brien Sr. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/3D-volume-rendering-and-measurement-tp3647068p3659152.html

One program that you might want to try is ImageSurfer.  It is free, which is a plus, and can do 3D rendering as an isosurface or volume rendering, and several color datasets can be shown together.  It has a really convenient slice extractor tool that lets you measure relationships between the datasets.  (It cannot measure volumes, per se, yet).  It is available from our CISMM website at UNC.  We are an NIBIB resource, so are interested in providing tools that Biologists (and other imaging scientists) will actually use. Download the program from  http://cismm.cs.unc.edu/downloads/.  There are also a number of other free tools for analyzing data from microscopy.  The software is constantly being updated vis a vis feedback from users.  It is good to have a good graphics card and a fair amount of ram memory when using it. 

Thanks,
Tim



firstname Joan Sim wrote:

Dear all, 
 
I am completely new to image analysis from confocal microscopy and I would 
like to seek advices about which softwares to use for 3D volume rendering and 
calculation of the volume after obtaining Z-stacks of immunostained Drosophila 
larvae brains. I briefly know that ImageJ (free) and Amira (expensive) are 
some of the available softwares, but can ImageJ perform 3D volume rendering 
and calculation? 
 
I would greatly appreciate any advices given. Thank you! 
 
Best regards,
Joan, PhD student
Singapore