Re: Fixation with frozen Paraformaldehyde

Posted by Cameron, Lisa on
URL: http://confocal-microscopy-list.275.s1.nabble.com/confocal-microscope-efficiency-measurement-tp3626645p3685404.html

In my post-doc lab, we used to make the fixation solution fresh, use it, and then freeze it at -20C once.

Anything left over after thawing it once was discarded.

If you typically use a certain volume of fixation, you could freeze aliquots of the appropriate size for maximal use.

 

Cheers -

Lisa

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Lisa Cameron
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Monday, September 21, 2009 9:35 AM
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] Fixation with frozen Paraformaldehyde

Bonjour à tous,

Can Paraformaldehyde 2-4% can be frozen for future use without lost of  fixation's efficiency?

Thanks for your help?

Louis

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