I routinely freeze 10mL aliquots of PFA
(freshly made from powder) 8% or 4%. I have used my stock without any problem
for 2 years.
I thaw a working stock that I keep in the
fridge for up to one month. I only warm up (if needed) the volume I need (so
the aliquot stays in the fridge) and add it 2x to the cell culture with the
same volume of warm medium. This perfectly preserves filopodia which are
fragile structures.
Med vänlig hälsning / Best regards
Sylvie
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Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240
From:
Sent: 21 September 2009 15:45
To:
[hidden email]
Subject: Re: Fixation with frozen
Paraformaldehyde
In my post-doc lab, we used to make the
fixation solution fresh, use it, and then freeze it at -20C once.
Anything left over after thawing it once
was discarded.
If you typically use a certain volume of
fixation, you could freeze aliquots of the appropriate size for maximal use.
Cheers -
Lisa
---------------------------------------
Lisa Cameron
Director of Confocal and Light Microscopy
Dana Farber Cancer Institute
From:
Sent: Monday, September 21, 2009
9:35 AM
To:
[hidden email]
Subject: [CONFOCALMICROSCOPY]
Fixation with frozen Paraformaldehyde
Bonjour à tous,
Can
Paraformaldehyde 2-4% can be frozen for future use without lost of fixation's
efficiency?
Thanks
for your help?
Louis
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