Re: DAPI on a 405 laser and 150x 1.45 objective

Posted by Colin Rickman-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/3D-volume-rendering-and-measurement-tp3647068p3693144.html

Hi,

I can't help for the first point but the 150x Olympus objective is a
fantastic objective. I have used it for TIRFM, 2D, 3D and 4D widefield
fluorescence microscopy, single molecule imaging and PALM. If you can
afford it and can live with the quite long lead time I can definitely
recommend it.

Regards

Colin

--
Dr Colin Rickman
Centre for Integrative Physiology
School of Biomedical Sciences
University of Edinburgh
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Renato Mortara wrote:

> Hello,
>  
> I wonder if I could get some feedback on how two doubts;
>  
> 1) How well does the solid-state 405 laser performs with DAPI-labeled
> samples (Cells, tissues, etc.) with not-so-UV-transparent objectives ?
>  
> I currently run an old BioRad 1024UV and with the Coherent 622 Inova
> laser (351/363 lines) even with  not-so-UV-transparent objectives such
> as a 63x PlanApo and a 100X 1.4 PlanApo (both from Zeiss), by using
> DAPI at a relatively high concentration (20-50 uM) we get very nice
> signal.
>  
> 2) Has anyone tried the 150x 1.45 NA Olympus objective (presumably
> designed for TIRF) with conventional/confocal fluorescence ? Is the
> small WD a problem ?
>  
>  
> Any input will be greatly appreciated,
>  
> Best regards,
>
> Renato
>  
> Renato A. Mortara
> Parasitology Division
> UNIFESP - Escola Paulista de Medicina
> Rua Botucatu, 862, 6th floor
> São Paulo, SP
> 04023-062
> Brazil
> Phone: [phone]
> Fax:     [phone]
> email: [hidden email] <mailto:[hidden email]>
> home page: www.ecb.epm.br/~ramortara

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