Re: DAPI on a 405 laser and 150x 1.45 objective
Posted by
Ghafar Sarvestani on
URL: http://confocal-microscopy-list.275.s1.nabble.com/3D-volume-rendering-and-measurement-tp3647068p3696694.html
Hello Renato,
We have a Coherent solid-state 405 laser in our Nikon
confocal microscope and we use it regularly for capturing images from
DAPI-labelled samples. It performs very well with Nikon 20x and 60x Plan Apo
Water objectives. Of course it depends
to the intensity level of Dapi-labelled samples and the level of
405 laser power you use.
Ghafar
***********************************
Ghafar T Sarvestani,
PhD
Detmold Imaging Centre
Hanson Institute/IMVS
T 08
8222 3603 M
040 25177123
F 08 8222
3162
IMVS
PO Box 14 Rundle Mall, Adelaide, SA,
5000
Quality Pathology supporting
Training and Research
Hello,
I
wonder if I could get some feedback on how two doubts;
1) How
well does the solid-state 405 laser performs with DAPI-labeled samples (Cells,
tissues, etc.) with not-so-UV-transparent objectives ?
I
currently run an old BioRad 1024UV and with the Coherent 622 Inova laser
(351/363 lines) even with not-so-UV-transparent objectives such as a 63x
PlanApo and a 100X 1.4 PlanApo (both from Zeiss), by using DAPI at a relatively
high concentration (20-50 uM) we get very nice signal.
2) Has
anyone tried the 150x 1.45 NA Olympus objective (presumably designed for TIRF)
with conventional/confocal fluorescence ? Is the small WD a problem ?
Any
input will be greatly appreciated,
Best
regards,
Renato
Renato A. Mortara
Parasitology Division
UNIFESP - Escola Paulista de
Medicina
Rua Botucatu, 862, 6th
floor
São Paulo, SP
04023-062
Brazil
Phone: 55 11 5579-8306
Fax: 55 11
5571-1095
home page:
www.ecb.epm.br/~ramortara