http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3782638.html
objective prism in our FV-1000/IX-81. It is also apparent while
looking at 6 um beads. Another reason it should be pulled from the
> Hi Mike,
>
> We have seen a similar problem to this, particularly apparent in DIC
> transmitted light images. However, of our two FV1000 systems
> (located in different rooms), it is only readily apparent on one.
> This has led us to believe it is a vibration issue, especially as we
> can cause a more severe, but similar-looking problem by deliberately
> introducing a source of vibration near to the microscope. However,
> if true, we have yet to isolate the cause of the problem vibration.
>
> While I'm here...has anyone properly investigated the effect of the
> DIC objective prism in confocal fluorescence imaging? I had always
> assumed (rightly or wrongly) that it's presence didn't influence the
> PSF, but last week I was imaging some subresolution beads and found
> that, particularly on our IX81-based FV1000 confocals, the DIC
> objective prism had quite a pronounced effect on the psf.
> Specifically the psf was distorted along a diagonal axis and at the
> point of focus, the bead appeared significantly larger with the
> prism in place. The implication of this is that for confocal
> fluorescence imaging, the resolution of the microscope is reduced
> when the DIC objective prism is in place. I've also looked on our
> Zeiss Axiovert 200 and Nikon TE-2000 based systems which employ a
> slightly different method of DIC and there the effect is much less
> pronounced although noticeable.
>
> Simon
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]] On Behalf Of MODEL, MICHAEL
> Sent: 07 October 2009 12:28
> To:
[hidden email]
> Subject: exorcising spirits from Fluoview
>
> I apologize if this is a second message to the list, I think the
> first one didn't go through
>
> We are having a bizzare scanning problem. Straight vertical lines in
> an object become slightly zigzagged with a period of up to 6-7 scan
> lines, and there also may be some oscillation in the intensity. The
> period and the magnitude of this periodic noise depends on the scan
> speed and the scan size. So far we (with the help of an Olympus
> engineer) have established that:
>
> 1. It doesn't seem to be the scanner controller or the galvo mechanism
> 2. It does not seem to be the electric power in the building
> 3. It is not a mechanical vibration
> 4. It is not a computer
> 5. It is not the cables
> 6. It is not a 60 Hz noise
>
> Sometimes connecting the scanner controller to the outlet through a
> long extension cord seemed to help which may suggest a problem with
> grounding, but as soon as we concluded that, the trick stopped
> working. The trouble could not be reproduced at the Olympus testing
> lab.
>
> Has anyone experienced anything similar and successfully resolved
> the problem?
>
> Many thanks in advance!
>
> Mike Model