optical clearing of tissue

Posted by Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3782683.html

Dear list,

I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use something
like that? Any input welcome.

cheers,
Michael