AW: optical clearing of tissue
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hello michael,
This publication might be interesting for you:
http://www.ncbi.nlm.nih.gov/pubmed/19397748thorsten
-----Ursprüngliche Nachricht-----
Von: Confocal Microscopy List im Auftrag von Michael Weber
Gesendet: Mi 07.10.2009 17:56
An:
[hidden email]
Betreff: optical clearing of tissue
Dear list,
I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use something
like that? Any input welcome.
cheers,
Michael