Posted by
Zucker.Robert on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3783264.html
Mike
This is a review that describes our procedure of clearing mammalian and
insect tissue with BABB. Reprints are available on request
Zucker, R.M.Technical note: Whole insects and Mammalian Embryo Imaging
with Confocal Microscopy: Morphology and Apoptosis. Cytometry 2006 69A:
1143-1152
Best wishes
bob
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Toxicology Assessment Division
Telephone: 919-541-1585 Fax: 919-541-4017
e-mail:
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Mail address: USEPA,ORD,NHEERL,TAD
Developmental Biology Branch ( MD 67)
Research Triangle Park, North Carolina, 27711
Shipping address:
2525 E.NC Highway 54
Durham, NC, 27713
From: Michael Weber <
[hidden email]>
To:
[hidden email]
Date: 10/07/2009 11:56 AM
Subject: optical clearing of tissue
Sent by: Confocal Microscopy List <
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Dear list,
I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use
something
like that? Any input welcome.
cheers,
Michael