Re: optical clearing of tissue

Posted by Phil Hertzler on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-optical-clearing-of-tissue-tp3783665.html

Mike,

Methyl salicylate (oil of wintergreen) also works well and smells better than BABB. :-) Transition through 100% ethanol from aqueous buffer.  I've stored samples over 15 years in MS without loss of fluorescence.

Best regards,

Phil

At 01:21 PM 10/7/2009, you wrote:

Mike
This is a review that describes our procedure of clearing mammalian and
insect tissue with BABB. Reprints are available on request

Zucker, R.M.Technical note: Whole insects and Mammalian Embryo Imaging
with Confocal Microscopy: Morphology and Apoptosis. Cytometry 2006 69A:
1143-1152

Best wishes
bob

Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Toxicology Assessment Division
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  From:       Michael Weber <[hidden email]>                                                                        
                                                                                                                      
  To:         [hidden email]                                                                        
                                                                                                                      
  Date:       10/07/2009 11:56 AM                                                                                     
                                                                                                                      
  Subject:    optical clearing of tissue                                                                              
                                                                                                                      
  Sent by:    Confocal Microscopy List <[hidden email]>                                             
                                                                                                                      





Dear list,

I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use
something
like that? Any input welcome.

cheers,
Michael

---

Philip L. Hertzler
Professor
Central Michigan University
Dept. of Biology, Brooks Hall 217
200 Library Dr.
Mount Pleasant, MI 48859

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