Posted by
Mario Faretta on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3787406.html
Dear List,
We are testing Chromomycin A3 as nuclear dye in alternative to DAPI in
mouse embryo fibroblasts. We noticed that the obtained staining pattern
looks quite different from the usual one we get.
Mouse cells usually have bright intense spots corresponding to
heterochromatin foci when stained with DAPI, but I have the impression
that the number of spots identified by ChromoA3 is less in number and
relative intensity. Do you know if according to the dye specificity we
have to expect this result (due to the relative distribution of AT or GC
bases)? (Cells were fixed (PAF) and permeabilized to reduce
accessibiltiy limitations)
Any suggestion/experience about nuclear dyes excited in the violet blue
range but with greater affinity for heterochromatin (Olivomycin,
Quinacrine Mustard, Syto Molecular Probes Dyes..) and feasible for DNA
quantitation (like cell cycle evaluation)?
Thanks
Mario
--
Mario Faretta
Department of Experimental Oncology
European Institute of Oncology
c/o IFOM-IEO Campus for Oncogenomics
via Adamello 16
20139 Milan
Italy
Phone: ++39-02574303054
email:
[hidden email]
http://www.ifom-ieo-campus.it