Re: optical clearing of tissue

Posted by Thomas Trusk on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3788221.html

We've had good success using the BABB procedure, described here:

http://www.ncbi.nlm.nih.gov/pubmed/16060974

Thomas C. Trusk, PhD
Josh Spruill Imaging Facility
Regenerative Medicine and Cell Biology
MUSC


-----Original Message-----
From: Michael Weber [mailto:[hidden email]]
Sent: Wednesday, October 07, 2009 11:56 AM
Subject: optical clearing of tissue

Dear list,

I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use something
like that? Any input welcome.

cheers,
Michael