Posted by
Stanislav Vitha on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3788369.html
Thorsten,
could you comment on the importance of TDE mountant purity?
Your article (MICROSCOPY RESEARCH AND TECHNIQUE 70:1–9, 2007) used the
Biochemica grade TDE (>99%, Sigma #88559, 250ML for $160.50). I have
been using the Aldrich cat. #166782 (>99%, 500g $27.70) with good success
for conofcal 3D imaging of pollen grains (autofluorescent, no staining needed).
It performed really well for these samples, but I am uncertain if this lower
grade TDE would work for e.g., immunostained slides.
Also, if anyone is using TDE with an antifade compound, I would appreciate
information what you used, what concentration, and any specific tips on
mixing.
Thank you!
Stanislav Vitha
Microscopy and Imaging Center
Texas A&M University
BSBW 119
College Station, TX 77843-2257
On Wed, 7 Oct 2009 18:10:56 +0200, Staudt Thorsten <T.Staudt@DKFZ-
HEIDELBERG.DE> wrote:
>hello michael,
>
>This publication might be interesting for you:
>
>
>
http://www.ncbi.nlm.nih.gov/pubmed/19397748>
>
>thorsten
>
>
>-----Ursprüngliche Nachricht-----
>Von: Confocal Microscopy List im Auftrag von Michael Weber
>Gesendet: Mi 07.10.2009 17:56
>An:
[hidden email]
>Betreff: optical clearing of tissue
>
>Dear list,
>
>I am looking for advice on optical "clearing" of fixed tissue before
>staining it and using it for light microscopy. Actually "tissue" is not
>the precise term, since I would like to clear whole fly embryos. This
>process seems to be well established in histology, i.e. using Xylene. I
>also found a commercial product called "Histo-Clear" (National
>Diagnostics), which claims to preserve tissue structures rather well,
>while being less nasty compared to Xylene. Did you guys ever use something
>like that? Any input welcome.
>
>cheers,
>Michael