Posted by
Stanislav Vitha on
URL: http://confocal-microscopy-list.275.s1.nabble.com/exorcising-spirits-from-Fluoview-tp3781225p3788421.html
We had a somewhat similar problem with our FV1000 a while ago, where
images would show a striped pattern, with orientation and period depending on
scan size and speed. It turns out that the fiber bringing the laser light from
the combiner/AOTF to the scan box was inserted too deep in the mount (or
something to that effect) and was touching or rubbing against the housing (I
do not remember exactly, it was few years ago). This caused vibrations and
consequently stripes in the images.
Stanislav Vitha
Microscopy and Imaging Center
Texas A&M University
BSBW 119
College Station, TX 77843-2257
On Wed, 7 Oct 2009 07:27:47 -0400, MODEL, MICHAEL <
[hidden email]>
wrote:
>I apologize if this is a second message to the list, I think the first one didn't
go through
>
>We are having a bizzare scanning problem. Straight vertical lines in an object
become slightly zigzagged with a period of up to 6-7 scan lines, and there also
may be some oscillation in the intensity. The period and the magnitude of this
periodic noise depends on the scan speed and the scan size. So far we (with
the help of an Olympus engineer) have established that:
>
>1. It doesn't seem to be the scanner controller or the galvo mechanism
>2. It does not seem to be the electric power in the building
>3. It is not a mechanical vibration
>4. It is not a computer
>5. It is not the cables
>6. It is not a 60 Hz noise
>
>Sometimes connecting the scanner controller to the outlet through a long
extension cord seemed to help which may suggest a problem with grounding,
but as soon as we concluded that, the trick stopped working. The trouble
could not be reproduced at the Olympus testing lab.
>
>Has anyone experienced anything similar and successfully resolved the
problem?
>
>Many thanks in advance!
>
>Mike Model