Re: Mattek dishes

Posted by Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mattek-dishes-tp3953839p3954256.html

<base href="x-msg://4/">Acid washing them before each use may help.  It is possible that you detergent does not clear all adherent protein and extracellular matrix off the glass surface.  

cheers, 

Tim Feinstein

On Nov 5, 2009, at 1:26 PM, Jennes, Mary E wrote:

They are purchased non-coated then etched in 50% Nitric acid overnight, Cleaned With Micro (detergent), series of EToH and Water rinses, then 30’ UV exposure.

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alice Rodriguez Diaz
Sent: Thursday, November 05, 2009 1:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

 

Hi,

 

Are they coated? I probably wouldn’t reuse them but if  did, I’d make sure I got the coating right… poly-lysine or other.

 

Best,

 

Alice Rodriguez-Diaz

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

 

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look “funny’ to me in that I can’t seem to get a crisp image. Does anyone have any experience with reusing dishes?

 

Thx!

 

Mary