Re: Mattek dishes

Posted by Alice Rodriguez Diaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mattek-dishes-tp3953839p3954306.html

Hi,

 

I believe it depends on what cells you are using, but they may just not tolerate the washing procedure… any type of residue will have an effect on adherence… this is one place where I wouldn’t try to cut corners…

 

Best,

 

Alice

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 12:27 PM
To: [hidden email]
Subject: Re: Mattek dishes

 

They are purchased non-coated then etched in 50% Nitric acid overnight, Cleaned With Micro (detergent), series of EToH and Water rinses, then 30’ UV exposure.

 


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alice Rodriguez Diaz
Sent: Thursday, November 05, 2009 1:07 PM
To: [hidden email]
Subject: Re: Mattek dishes

 

Hi,

 

Are they coated? I probably wouldn’t reuse them but if  did, I’d make sure I got the coating right… poly-lysine or other.

 

Best,

 

Alice Rodriguez-Diaz

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jennes, Mary E
Sent: Thursday, November 05, 2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

 

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look “funny’ to me in that I can’t seem to get a crisp image. Does anyone have any experience with reusing dishes?

 

Thx!

 

Mary