Re: Mattek dishes

Posted by Rossi, Ann [BSD] - MED on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mattek-dishes-tp3953839p3954885.html

I've reused uncoated Mattek dishes for primary skeletal muscle fibers used the same day as they were isolated.  I found that a quick sponge wash with Alconox followed by a rinse in distilled tap water and then a 70% ethanol rinse worked quite well.  But, I only could wash the dishes twice before subsequent fiber preps would stop adhering to the glass.  Never had a problem with the "crispness" of images, as long as the cells stuck to the dish.


Ann E. Rossi, Ph.D.
Postdoctoral Fellow
University of Chicago
Department of Medicine



-----Original Message-----
From: Confocal Microscopy List on behalf of Jennes, Mary E
Sent: Thu 11/5/2009 11:46 AM
To: [hidden email]
Subject: Mattek dishes

Has anyone ever reused Mattek dishes? I have a user who is trying to save some money and is cleaning their dishes with a detergent, rinsing and exposing to UV light. The cells look "funny' to me in that I can't seem to get a crisp image. Does anyone have any experience with reusing dishes?

Thx!

Mary

 

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