Posted by
Richard Berman on
URL: http://confocal-microscopy-list.275.s1.nabble.com/UV-lines-on-Yokogawa-CSU-X1-Head-tp3985884p3987696.html
Dear Renato:
Your timing is pretty good. Over the next few months we intend to
explore the potential of using the Yokogawa CSU at 375nm. For our
testing we will use the CSU-10 although the results should apply to the
X1 as well. Although the wavelength is not as low as 355nm, it is lower
than any previous work of which we are aware. You are correct to
question the transmission of the disks but there are other concerns too,
mostly on the excitation path. The transmission of the standard fiber
will be very poor at 375nm and could have a shorter lifetime when used
with such low wavelengths. We will investigate other fiber materials. We
are concerned that the chromatic shift in the collimating lenses and the
lenslets themselves could introduce problems. In addition, there are
the usual concerns about the transmission efficiency of the scope and
objectives. A special CSU dichroic will be required to make this
possible too.
From our side we would like to hear from you or other CSU users about
the potential benefits of 375nm excitation light. For your application
is it necessary to get down to 355nm or is 375nm useable? Operating at
355nm may be possible although it is not on our current test plans.
Regards, Richard
--
Richard Berman
[hidden email]
Spectral Applied Research
9078 Leslie St., Unit 11
Richmond Hill, Ontario
L4B 3L8
Renato Mortara wrote:
> Hi !
>
> Does any one has experienced using UV lines , 353 and/or 363 nm with
> the Yokogawa CSU-X1 Head. Yokogawa claim that in the range of 400 -
> 700 their microlenses work fine, but have no experience with lower
> wavelengths.
>
> Any input (pros + cons) will be greatly appreciated.
>
> All the best
>
> Renato
>
> Renato A. Mortara
> Parasitology Division
> UNIFESP - Escola Paulista de Medicina
> Rua Botucatu, 862, 6th floor
> São Paulo, SP
> 04023-062
> Brazil
> Phone: 55 11 5579-8306
> Fax: 55 11 5571-1095
> email:
[hidden email] <mailto:
[hidden email]>
> home page: www.ecb.epm.br/~ramortara
--
Richard Berman
Spectral Applied Research
9078 Leslie St., Unit 11
Richmond Hill, Ontario
L4B 3L8
905-326-5040 ext. 444
www.spectral.ca