Re: UV lines on Yokogawa CSU-X1 Head

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/UV-lines-on-Yokogawa-CSU-X1-Head-tp3985884p3988036.html

Hi

To raise another concern, what about  UV damage to the micro lenses?

Mark.

Richard Berman wrote:

> Dear Renato:
>
> Your timing is pretty good. Over the next few months we intend to
> explore the potential of using the Yokogawa CSU at 375nm. For our
> testing we will use the CSU-10 although the results should apply to
> the X1 as well. Although the wavelength is not as low as 355nm, it is
> lower than any previous work of which we are aware. You are correct to
> question the transmission of the disks but there are other concerns
> too, mostly on the excitation path. The transmission of the standard
> fiber will be very poor at 375nm and could have a shorter lifetime
> when used with such low wavelengths. We will investigate other fiber
> materials. We are concerned that the chromatic shift in the
> collimating lenses and the lenslets themselves could introduce
> problems.  In addition, there are the usual concerns about the
> transmission efficiency of the scope and objectives. A special CSU
> dichroic will be required to make this possible too.
>
> From our side we would like to hear from you or other CSU users about
> the potential benefits of 375nm excitation light. For your application
> is it necessary to get down to 355nm or is 375nm useable? Operating at
> 355nm may be possible although it is not on our current test plans.
>
> Regards, Richard
>