Re: UV lines on Yokogawa CSU-X1 Head

Posted by Gary Yellen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/UV-lines-on-Yokogawa-CSU-X1-Head-tp3985884p3992829.html

For using UV excitation with the SD (or in any confocal scope), I would be
concerned about the chromatic aberration in the objectives.  Some of the new
objectives from many mfrs are now corrected down to 405 nm; most objectives
are not even this good.  Even without the problem of % transmission in the
UV, if the excitation wavelength is focused in a different plane than the
emission wavelength, the pinhole will reject much of the emitted light.