Posted by
Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/UV-lines-on-Yokogawa-CSU-X1-Head-tp3985884p4002677.html
I don't really know how the microlenses are made, but I assume it's by
some semiconductor-type process. In which case, they might well be
silica, which will transmit UV OK. Whatever they are, it would seem
unlikely that they have any chromatic correction. I'd guess that they
would be designed around 488nm, so 365 would be out by 123nm -
equivalent to 611 in the visible direction. Thus if the system will
work with a He-Ne (635) it should also work with 365.
As Gary says, the objective is likely to be a problem, but you can buy
violet corrected objectives which should help quite a bit. The other
snag is that these fancy objectives tend to be 60x, whereas I understand
the Yokogawa head is designed around 100x. Confocality will therefore
be worse at 60x (pinholes too large) but on the flip side there will be
a bit more latitude for aberrations.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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http://www.guycox.net-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Gary Yellen
Sent: Friday, 13 November 2009 12:37 AM
To:
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Subject: Re: UV lines on Yokogawa CSU-X1 Head
For using UV excitation with the SD (or in any confocal scope), I would
be concerned about the chromatic aberration in the objectives. Some of
the new objectives from many mfrs are now corrected down to 405 nm; most
objectives are not even this good. Even without the problem of %
transmission in the UV, if the excitation wavelength is focused in a
different plane than the emission wavelength, the pinhole will reject
much of the emitted light.
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