I agree, Nikons Perfect Focus works really well and I think it’s
must for any long term time lapse experiments. I also forgot to mention that all
are systems are Windows XP, only the offline post processing workstation is
Windows Vista.
Neeraj.
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Neeraj Gohad
Sent: Wednesday, November 18, 2009 1:13 PM
To: [hidden email]
Subject: Re: NIS elements software
Hi Valeria,
We too have NIS elements on our motorized Nikon TiE microscope
as well as on the Nikon AZ-100 and UDM microscope. We also have an
offline post-processing workstation. As Evangelos suggested you can add modules
and features as per your need. On the microscope control side, setting up
multidimensional experiments is really easy, I routinely conduct 3-4 day
time lapse experiments on taking images every couple of minutes on different
stage points in different channels. Setting up different optical configuration
such as objectives and filter cubes and assigning microscope and camera setting
to theses configurations is also straightforward and intuitive. For
analysis, you can trim down datasets and easily extract specific time points,
focal planes, channels etc. There is wide variety of annotation and measurement
tools. One of the features which comes handy is merging of different
channels, you just have to drag and drop to get a multichannel
image. In all I think it’s a good software for both control and analysis,
but there some room for improvement with the free image viewer, it should do
lot more than what it does.
As always, no commercial interests.
Best,
Neeraj.
Neeraj V.
Gohad, Ph.D.
Postdoctoral
Fellow
Okeanos
Research Group
Department of
Biological Sciences
132 Long Hall
Clemson
University
Clemson,SC-29634
Phone:
864-656-3597
Fax:
864-656-0435
Please note
my new email address: [hidden email]
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Marco Dal
Maschio
Sent: Wednesday, November 18, 2009 10:16 AM
To: [hidden email]
Subject: Re: NIS elements software
Dear Valeria,
we currently work with NIS controlling a motorized widefield
TiE microscope and a hamamatsu camera
for acquisition of Ca signal but also for multicolor cell
immunostaining that you can perform in full automatic mode
with a macro. We tested the same software with the A1
confocal (same microscope with XY sample holder traslator): very reliable.
Still missing a relaible integration with UNIBLITZ shutter.
No complain at all!
If you need more details do not esitate to contact me
My best
Marco
On Wed, Nov 18, 2009 at 3:36 PM, Valeria Berno <[hidden email]> wrote:
Hi
did any of you have experience with NIS element software from Nikon?
Any complain?
quick check in order to take a decision for a widefield microscope....
thanks in advance
Valeria
Berno Valeria, PhD
EMBL- Mouse Biology Unit
Campus A. Buzzati-Traverso
Via Ramarini, 32
00015, Monterotondo Scalo (RM)
Italy
Tel: +39 06 90091243
Fax: +39 06 90091406
email: [hidden email]
www.embl.it
--
Marco Dal Maschio [hidden email]
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