Re: NIS elements software

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/NIS-elements-software-tp4025804p4028158.html

We have NIS elements for Windows XP and it works fine.  I would be
suspicious of the version for Vista; maybe the Windows 7 version will
be more solid.  Our university has avoided vista entirely and still
only offers windows XP as its license.  I expect we will skip Vista
entirely and eventually switch to Windows 7.
We don't have Perfect Focus yet but we are thinking of upgrading based
on comments from various users (both on this list and others).  It
sounds like a solid performer.

Craig


On Wed, Nov 18, 2009 at 12:25 PM, Neeraj Gohad <[hidden email]> wrote:

> I agree, Nikons Perfect Focus works really well and I think it’s must for
> any long term time lapse experiments. I also forgot to mention that all are
> systems are Windows XP, only the offline post processing workstation is
> Windows Vista.
>
>
>
> Neeraj.
>
>
>
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Neeraj Gohad
> Sent: Wednesday, November 18, 2009 1:13 PM
> To: [hidden email]
> Subject: Re: NIS elements software
>
>
>
> Hi Valeria,
>
>
>
> We too have NIS elements on our motorized Nikon TiE microscope as well as on
> the Nikon AZ-100 and UDM microscope. We also have  an offline
> post-processing workstation. As Evangelos suggested you can add modules and
> features as per your need. On the microscope control side, setting up
> multidimensional experiments is really easy, I routinely  conduct 3-4 day
> time lapse experiments on taking images every couple of minutes on different
> stage points in different channels. Setting up different optical
> configuration such as objectives and filter cubes and assigning microscope
> and camera setting to theses configurations is also straightforward and
> intuitive.  For analysis, you can trim down datasets and easily extract
> specific time points, focal planes, channels etc. There is wide variety of
> annotation and measurement tools. One of the features which comes handy  is
> merging of different channels, you  just have to  drag and drop to get a
> multichannel image.  In all I think it’s a good software for both  control
> and analysis, but there some room for improvement with the free image
> viewer, it should do lot more than what it does.
>
>
>
> As always, no commercial interests.
>
>
>
> Best,
>
>
>
> Neeraj.
>
>
>
>
>
> Neeraj V. Gohad, Ph.D.
>
> Postdoctoral Fellow
>
> Okeanos Research Group
>
> Department of Biological Sciences
>
> 132 Long Hall
>
> Clemson University
>
> Clemson,SC-29634
>
> Phone: [phone]
>
> Fax: [phone]
>
>
>
> Please note my new email address: [hidden email]
>
>
>
>
>
>
>
>
>
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Marco Dal Maschio
> Sent: Wednesday, November 18, 2009 10:16 AM
> To: [hidden email]
> Subject: Re: NIS elements software
>
>
>
> Dear Valeria,
>
> we currently work with NIS controlling a motorized widefield TiE microscope
> and a hamamatsu camera
>
> for acquisition of Ca signal but also for multicolor cell immunostaining
> that you can perform in full automatic mode
>
> with a macro. We tested the same software with the A1 confocal (same
> microscope with XY sample holder traslator): very reliable.
>
> Still missing a relaible integration with UNIBLITZ shutter.
>
>
>
> No complain at all!
>
>
>
> If you need more details do not esitate to contact me
>
> My best
>
> Marco
>
>
>
>
>
>
>
> On Wed, Nov 18, 2009 at 3:36 PM, Valeria Berno <[hidden email]>
> wrote:
>
> Hi
>
> did any of you have experience with NIS element software from Nikon?
>
> Any complain?
>
> quick check in order to take a decision for a widefield microscope....
>
> thanks in advance
>
> Valeria
>
> Berno Valeria, PhD
>
> EMBL- Mouse Biology Unit
> Campus A.  Buzzati-Traverso
> Via Ramarini,  32
> 00015, Monterotondo Scalo  (RM)
> Italy
>
> Tel:  [phone]
> Fax: [phone]
> email: [hidden email]
> www.embl.it
>
>
> --
> Marco Dal Maschio      [hidden email]
> Home: via Garzetta 23
>          30015 Chioggia VE
>           mob:  [phone]
>
>