Re: Big Tiff (.btf) file format

Posted by Shalin Mehta on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Big-Tiff-btf-file-format-tp4087167p4087222.html

Hi Oliver,
If you are using 32-bit version of ImageJ that may  not be able to
load files larger than 4 GB. 64-bit ImageJ should be able to do so. I
think when RAM is low, one can use virtual stacks to load only those
slices that are being processed. LOCI bioformats  supports files from
CellR but no mention of big-tiffs.
http://www.loci.wisc.edu/bio-formats-format/olympus-cellrapl
A post here  suggests that big tiffs are supported:
http://n2.nabble.com/Opening-btf-files-td4074793.html

hope this helps
Shalin


On Mon, Nov 30, 2009 at 7:42 PM, Oliver Biehlmaier
<[hidden email]> wrote:

> Hi everyone,
>
> Our Olympus CellR system generates “Big Tiff”-files (.btf) instead of
> conventional Tiff-files if a multidimensional acquisition experiment exceeds
> 4GB.
> Bitplane’s Imaris does not recognize the btf. ImageJ tries to open but
> freezes...
> Does anyone know which programs can open and handle these files?
>
> Many thanks in advance,
> Oliver
>
>
> <><><><><><><><><><><><><><><><><><><><><><><><>
>
> Oliver Biehlmaier, PhD
>
> Advanced Microscopy
> Light Microscopy Centre (LMC)
> Schafmattstr. 18
> ETH-Hönggerberg, HPM F16
> 8093 Zürich
> Switzerland
>
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