Re: Big Tiff (.btf) file format

Posted by Rickman, Colin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Big-Tiff-btf-file-format-tp4087167p4089425.html

Hi Oliver

I have had similar issues with Bigtiff files from Olympus CellR. I
posted to the ImageJ list last week and Curtis Rueden (OME Loci) is
having a look at why I can't open them using the Bioformats plugin. My
solution at present is to use the CellR software to split the file in to
a tif image sequence and load the sequence in ImageJ (64bit Java in
Linux with 6Gb RAM allocated). Imaris should also be able to import the
sequence (on a 64bit computer with plenty of RAM) but you will have to
check the channel, Z and T dimensions after import .

Regards

Colin

> On Nov 30, 2009, at 3:42 AM, Oliver Biehlmaier wrote:
>
>> Hi everyone,
>>
>> Our Olympus CellR system generates “Big Tiff”-files (.btf) instead of
>> conventional Tiff-files if a multidimensional acquisition experiment
>> exceeds 4GB.
>> Bitplane’s Imaris does not recognize the btf. ImageJ tries to open
>> but freezes...
>> Does anyone know which programs can open and handle these files?
>>
>> Many thanks in advance,
>> Oliver
>>
>>
>> <><><><><><><><><><><><><><><><><><><><><><><><>
>>
>> Oliver Biehlmaier, PhD
>>
>> Advanced Microscopy
>> Light Microscopy Centre (LMC)
>> Schafmattstr. 18
>> ETH-Hönggerberg, HPM F16
>> 8093 Zürich
>> Switzerland
>>
>> Phone:  [phone]
>> Fax:    [phone]
>> Email:  [hidden email]
>> URL:    http://www.lmc.ethz.ch/People/Oliver
>> <><><><><><><><><><><><><><><><><><><><><><><><>
>>
>

--
--
Dr Colin Rickman
School of Engineering and Physical Sciences Heriot-Watt University Edinburgh
EH14 4AS

Tel: [phone]
Fax: [phone]