Re: Spinning disk requirement

Posted by anurag pandey on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-requirement-tp4231297p4232496.html

 Dear Charu
 Do you really need very fast live cell acquisition, as for as i know the only
benefits of DSUs is that you can scan at a very fast rate but you can still
achieve at least 250-300 frames per second by any good resonance scanner. I
have seen both Ziess and Olympus DSUs i did not find anything great other than
speed if one take the huge prices in account. one good thing about these
systems is low noise, as they have CCD camera but this reduces sensitivity.


On Wed, December 30, 2009 5:19 pm, charu tanwar wrote:

> No, i have not yet seen any of the spinning disk systems. Yokogawa is very
> famous but i really want to know that how reasonable is to buy a laser based
> multipoint scanning system than buying fluorescence based spinning disk. (we
> already have single point scanning system, one only confocal and another
> confocal with live cell, both laser based.)
>
> --- On Wed, 30/12/09, Vergara, Leoncio A. <[hidden email]> wrote:
>
>
> From: Vergara, Leoncio A. <[hidden email]>
> Subject: Re: Spinning disk requirement
> To: [hidden email]
> Date: Wednesday, 30 December, 2009, 4:12 PM
>
>
> have you tested the Olympus DSU? I am wondering how it would compare with the
> yokowaga scanhead based systems.
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On Behalf Of
> Charu Tanwar [[hidden email]]
> Sent: Wednesday, December 30, 2009 1:36 AM
> To: [hidden email]
> Subject: Spinning disk requirement
>
> Dear All
>
> I request all the members to please help me to decide for a spinning disk
> confocal microscope for our facility which is a multiuser facility for the
> whole university. We already have a point scanning confocal system from
> Olympus. We will have another point scanning confocal soon with all the live
> cell imaging accessories (laser based).
> For very fast phenomenon like measuring Calcium Flux in the cells, we want
> to have a multi point scanning system. But i am unable to decide whether i
> should take a fluorescence based spinning disk or laser based spinning disk
> confocal.
> Also, please give me some feedback for CARV II (multi point confocal,
> fluorescence based).
> Please help.
> Thanks in advance.
>
> Charu Tanwar
> Imaging Specialist
> Advanced Instrumentation Research Facility
> Jawaharlal Nehru University
> New Delhi
> India.
>
>
>
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--
ANURAG PANDEY
Electro physiology Group
M.B.U.
I.I.Sc.



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