Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-requirement-tp4231297p4232702.html
Hi Charu,
Check out VT-Infinity and VT-Hawk at Visitech
http://www.visitech.co.uk/site/products.php The VT-Hawk adds
FRAP/photoactivation capability to the Infinity. Visitech sold
Yokogawa's for a long time - Steve Coleman told me in April 2009 that
their company was selling off its remaining spinning disk inventory
to focus exclusively on their array scanners. A VT with the right
EMCCD would be a great combination. My biggest concern with Visitech
is how do they support overseas: they are a British company, so I am
referring to both here (USA) and you.
As for Calcium ion flux, the "standard of care" is Fura-2 for its
dynamic range. However, the excitation light path of most
fluorescence microscopes are such that UV is usually not parfocal
with visible. Until recently, even violet (405 nm) was not parfocal
with green and red (and now only a few expensive objective lenses
are). Parfocality is not critical in widefield Fura-2, but would be
for confocal. The dynamic range of fluorescent protein based
biosensors is not anywhere near Fura-2, but are getting better, for
example, GCaMP-3, and can be targeted to specific location(s) by
appropriate targeting sequences.
CARV II - I manage a BD pathway 855 that has a CARV (I?) inside. Nice
to be able to see confocal by eye, but the BD (formerly ATTO)
software is bad. If you buy a CARV II, be sure to evaluate the
software that you will be using with it.
Sincerely,
George
At 02:36 AM 12/30/2009, you wrote:
>Dear All
>
>I request all the members to please help me to decide for a spinning disk
>confocal microscope for our facility which is a multiuser facility for the
>whole university. We already have a point scanning confocal system from
>Olympus. We will have another point scanning confocal soon with all the live
>cell imaging accessories (laser based).
>For very fast phenomenon like measuring Calcium Flux in the cells, we want
>to have a multi point scanning system. But i am unable to decide whether i
>should take a fluorescence based spinning disk or laser based spinning disk
>confocal.
>Also, please give me some feedback for CARV II (multi point confocal,
>fluorescence based).
>Please help.
>Thanks in advance.
>
>Charu Tanwar
>Imaging Specialist
>Advanced Instrumentation Research Facility
>Jawaharlal Nehru University
>New Delhi
>India.
George McNamara, Ph.D.
Image Core Manager
Analytical Imaging Core Facility
University of Miami, Miller School of Medicine
Miami, FL 33136
[hidden email]
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http://www.sylvester.org/AICF (Analytical Imaging Core Facility)
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