Re: Spinning disk requirement

Posted by Michael Herron on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-requirement-tp4231297p4233215.html

I just acquired a DSU for live cell imaging.  If price is no object  
the laser systems are swell but if you are on a budget I think the DSU  
is a reasonable device.  It is my understanding that the CARVII is at  
end of life. As for sensitivity, a CCD is a good bet.

Another nice aspect of a non-laser system is the flexibility of a  
white light source.  You never know if the next great fluor will meet  
you laser lines. This might be esp the case in a multiuser set up.


On Dec 30, 2009, at 1:36 AM, Charu Tanwar wrote:

> Dear All
>
> I request all the members to please help me to decide for a spinning  
> disk
> confocal microscope for our facility which is a multiuser facility  
> for the
> whole university. We already have a point scanning confocal system  
> from
> Olympus. We will have another point scanning confocal soon with all  
> the live
> cell imaging accessories (laser based).
> For very fast phenomenon like measuring Calcium Flux in the cells,  
> we want
> to have a multi point scanning system. But i am unable to decide  
> whether i
> should take a fluorescence based spinning disk or laser based  
> spinning disk
> confocal.
> Also, please give me some feedback for CARV II (multi point confocal,
> fluorescence based).
> Please help.
> Thanks in advance.
>
> Charu Tanwar
> Imaging Specialist
> Advanced Instrumentation Research Facility
> Jawaharlal Nehru University
> New Delhi
> India.

Michael J. Herron,  U of MN, Dept. of Entomology
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