Thanks for the clarification. The source of my information was a
little over a year ago when a group on our campus (UTMB, Galveston) had a demo
of a Nikon swept field, from the talk given by the rep and the literature
distributed I understood the Nikon system is a dual system that uses either a set
of parallel slits or a pinhole array similar to the Visitech system. The
same group also run a demo on a Yokogawa based system. The decision was in
favor of the Nikon system (the purchase later did not materialize, in part due
to Ike I think), nevertheless it is my impression the choice was heavily based
on the circumstantial performance of both the demo systems and the company
teams, one of the faculty even told me they did not like the incubator
included with the Yokogawa system (what about the color of the eyes of the
sales rep?). I am convinced that these kind of demos need to be taken with a
grain of salt as there are many acute factors that can influence the outcome. At
the medical center in Houston I met another group that purchased the same Yokogawa
system that was tested here, they also considered the swept filed system but
their opinion was just the opposite, they purchased the spinning disk system
and are very happy with the performance.
The information in this thread has been very useful for me, in
the future if we are again ready to evaluate a fast confocal system for
live cell imaging I will have all these comments in mind.
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of RICHARD
BURRY
Sent: Monday, January 04, 2010 4:20 PM
To: [hidden email]
Subject: Re: Spinning disk requirement ... consider alternative array
scan confocal
Leoncio
The Visitech Infinity system array scanner is not swept field. It
uses arrays of seven different sizes of pinholes from 64 to 10
microns and no slits. The technology for the light path is unique.
In my experience the through put of the Infinity is better and the optical
section thickness is finer.
Dick
----- Original Message -----
From: "Vergara, Leoncio A." <[hidden email]>
Date: Monday, January 4, 2010 12:19 pm
Subject: Re: Spinning disk requirement ... consider alternative array scan
confocal
To: [hidden email]
|
> Array scanners are sold in the
US by Nikon and Prairie Technologies. I am not sure if there are other
sources and how they compare to the Visitech systems. I have no personal
experience with eithe of the systems but I thought the information may
help. > There are always trade offs with
any design, Yokogawa scanheads have been around for years and I
understand the design has been improved with the new models. The array
scanners are however realtively new and untried. > I think
you should test the systems and compare by yourself. The best is to
get a system to test at your site for an extended time is the ideal situation
but difficult to get. I am not convinced that short term demos are
the best since many times you may get biased by the skills of the
demo crew or you may have problems with a system due to precarious
installations or technical problems... the second best is to travel to a
location were a system has been in operation for some time, there you also
can also talk to experienced users. The problem of traveling to a
distant location is that your live specimen preparations may not travel
well. > From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of charu
tanwar
> The
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Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
277 Biomedical Research Tower
460 West Twelfth Avenue
Columbus, Ohio 43210
Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849
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