Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p4283953.html
I bought a high-powered LED meant for architectural lighting (normally
put in a spotlight outside a building) and duct-taped it (literally)
onto the side of an old microscope we found in a junk heap with no
lamp. I put a cheap condenser lens in front of the LED array to
collimate it with pretty decent results. The LED is actually an array
of RGBA elements (red, green, blue, amber) so I have four wavelengths
to choose from. Control is provided by a USB connection to a PC. It
works well enough for our cell culture work, where we just want a
quick check of whether or not a given cell stained properly. We use
epi-fluorescene using the LEDs. The one drawback of them is the blue
LED is centered more at 460nm, so it is not so great for some dyes
like CFP, but it works well for other dyes. In particular, the Amber
LED is 590, which works well for m-cherry (I believe it is what they
were using).
Craig
On Sun, Jan 10, 2010 at 8:20 PM, James Pawley <
[hidden email]> wrote:
>> Hi,
>>
>> I was wondering if anybody has some experience with using LEDs as
>> excitation sources for fluorescence microscopy and could recommend me some
>> companies and/or specific models.
>>
>> We are interested in equipping a Nikon Ti with LEDs instead of the
>> conventional mercury lamp (e.g. Nikon's Intensilight). The main application
>> of the microscope would be quantitative high-throughput time-lapse
>> microscopy.
>>
>> Thanks a lot. Dirk
>
>
> A good place to start might be Chapter 6 of The Handbook of Biological
> Confocal Microscopy.
>
> Cheers,
>
> Jim P.
>
> --
> **********************************************
> Prof. James B. Pawley, Ph.
> 608-263-3147 Room 223, Zoology Research Building, FAX
> 608-265-5315
> 1117 Johnson Ave., Madison, WI, 53706
[hidden email]
> 3D Microscopy of Living Cells Course, June 12-24, 2010, UBC, Vancouver
> Canada
> Info:
http://www.3dcourse.ubc.ca/ Applications due by March 15,
> 2010
> "If it ain't diffraction, it must be statistics." Anon.
>