Re: LEDs

Posted by Straatman, Kees (Dr.) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4283200p4284789.html

Dear Dirk,

Here in Leicester we have a Nikon Ti with COOLLED PrecisExcite High Power LED Fluorescent Excitation System, using 400, 490 and 565 nm LEDs and EM-CCD. The LED system was bought with the microscope via Nikon. The system is mostly used for yeast imaging and gives very good results, due to reduced photo bleaching, as compared to our Leica SP5 confocal.

Best wishes

Kees

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Dirk Landgraf
Sent: 11 January 2010 00:22
To: [hidden email]
Subject: LEDs

Hi,

I was wondering if anybody has some experience with using LEDs as  
excitation sources for fluorescence microscopy and could recommend me  
some companies and/or specific models.

We are interested in equipping a Nikon Ti with LEDs instead of the  
conventional mercury lamp (e.g. Nikon's Intensilight). The main  
application of the microscope would be quantitative high-throughput  
time-lapse microscopy.

Thanks a lot. Dirk