Live cell imaging experiences, please

Posted by Zoltan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-imaging-experiences-please-tp4285373.html

Dear All,

We are planning to run a few tests, where we follow cell development
in 8-9 XY positions, running 45 um Z stacks (step size 2.5 um), 2.5
min time interval, for a total of 14 hours at room temp, using GFP and
DsRed settings.  We had lots of success with the Leica SP5 system for
the past 2 years.  We are wondering if this community has more input
about how other systems would perfom here?  We are most interested in
Olympus FV1000 and Nikon A1.  Our main concerns are the simplicity of
the setting up procedure, the stability of the system during the long
scans, and the handling of the large data files.
  All inputs will be much appreciated!  Happy New Year to all!  With
best wishes,

Zoltan

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Zoltan Cseresnyes
Facility manager, Imaging Suite
Univ. Cambridge, UK