LEDs

Posted by Richard Cole on
URL: http://confocal-microscopy-list.275.s1.nabble.com/LEDs-tp4288043.html

LEDs offer many advantages over traditional sources such as mercury arc lamp.

 

Most notably, they provide brightness and spectral control.  For fluorescent imaging, LEDs offer advantages of providing longer lifespan, higher spatial and temporal stability, eliminate the need for mechanical shutters, neutral density filters, significantly lower cost of ownership and reduce photon dose at the specimen. Additionally, LEDs allow for vibration free high speed spectral and temporal modulation. 

 

There are many commercial vendors:

 

The Colibri illumination system (Zeiss Optics Inc. Thornwood, NY 10594) offers up to ten different LEDs that can be switched on and off and be adjusted in the microsecond range solely based on optoelectronics. 

 

There are many aftermarket sources for LED based illumination systems.  An example of complete illumination system is the CoolLED system (CoolLED,  Andover, UK) with up to 18 different LED wavelengths to match excitation peaks of fluorophores available in manual or automatic control modes.  There are many modular LED based illumination systems available; the Prizmatix (Prizmatix Ltd., Southfield, MI 48075) is one example, comprised of up to three high power LED modules and corresponding dichroic beam splitters.  Modulated LED Source, such as model DC3100, applicable for live cell imaging or fluorescence lifetime imaging (FLIM) microscopy is available from Thor Labs (Newton, New Jersey 07860) and adaptable for most microscope stands.  

 

 

There are even more component level sources

 

Regards,

 

Rich

 

 

I have no commercial interest in any of the aforementioned companies

 

 

Richard Cole
Research Scientist IV
Director: Advanced Light Microscopy Core Unit
Wadsworth Center

 

Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York

P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax

 

Email [hidden email]

Website www.wadsworth.org/cores/alm/index.htm

 

 

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CONFOCALMICROSCOPY Digest - 8 Jan 2010 to 10 Jan 2010 (#2010-4) Table of contents: LEDs (3) LEDs <a href="cid:[e-mail]">LEDs (01/10) From: Dirk Landgraf <[hidden email]> <a href="cid:[e-mail]">Re: LEDs (01/10) From: James Pawley <[hidden email]> <a href="cid:[e-mail]">Re: LEDs (01/10) From: Craig Brideau <[hidden email]>

 

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